5-Methylcytosine / 5-mC antibody [GT4111]

Cat. No. GTX629448

GTX629448 MeDIP Image

GTX629448 5-Methylcytosine/ 5-mC antibody [GT4111] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody [GT4111] (GTX629448) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

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GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebral cortex.
5-Methylcytosine / 5-mC stained by 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-Methylcytosine / 5-mC stained by 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX629448 IHC-Wm Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.

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GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleolus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX629448 IHC-Wm Image

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GTX629448 Dot Image

GTX629448 5-methylcytosine antibody [GT4111] Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (GTX629448) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

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This 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) is a mouse monoclonal antibody validated for detection of 5-Methylcytosine / 5-mC by IHC-P, IHC-Fr, IHC-Wm, Dot, EM, and MeDIP. Reactivity is species-independent. This product is cited in 12 published studies.

Host

Mouse
Clonality

Monoclonal
Clone Name

GT4111
Isotype

IgG1
Applications

IHC-P IHC-Fr IHC-Wm Dot EM MeDIP
Reactivity

Species independent, Mouse

Summary

5-mC antibody detects methylated cytosine, which is a key epigenetic regulatory modification of genomic DNA and is considered a “fifth base”. The 5-mC mark is added by DNA methyltransferases (DNMTs) and serves predominately to turn off gene transcription. It is reversible via a DNA demethylation pathway triggered by the Ten-eleven translocation (TET) family of dioxygenases.