CD5 antibody [HL5247]

CD5 antibody [HL5247] (GTX645503) detects CD5 protein by flow cytometry analysis.
Sample: Jurkat were blocked with an FcR blocking reagent for 10 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer to permeabilize the cell membrane.
The cells were following stained with CD5 antibody [HL5247] (GTX645503)diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) control at 4ºC for 1 hour.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD5 antibody [HL5247] (GTX645503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.