Cyclin D1 antibody [HL5522]

Cyclin D1 antibody [HL5522] detects Cyclin D1 protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Cyclin D1 stained by Cyclin D1 antibody [HL5522] (GTX646185) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield™ with DAPI (GTX30920).

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [HL5522] (GTX646185) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.