GeneTex
EasyBlot anti Mouse IgG (HRP)
Cat. No. GTX221667-01
Cat. No. GTX221667-01
GTX221667-01 WB Image
EasyBlot anti-Mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent for immunoprecipitation analysis. 293T lysates were immunoprecipitated using 5 ug of normal mouse IgG or ELP3 antibody [GT11811] (GTX631395)
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GTX221667-01 WB Image
Western Blot analysis of native mouse IgG (left) or denatured mouse IgG (right) using EasyBlot anti Mouse IgG (HRP) antibody.
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The EasyBlot anti Mouse IgG (HRP) (GTX221667-01) is a HRP-conjugated goat polyclonal secondary antibody that detects Mouse IgG. It is validated for WB. This product is cited in 55 published studies.
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HostGoat
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ClonalityPolyclonal
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IsotypeIgG
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ApplicationsWB
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ReactivityMouse
Summary
EasyBlot anti-Mouse IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of mouse IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to mouse IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian.
To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Mouse IgG (Optimized for Prot A/G) (GTXGTX225857-01) or EasyBloker (GTX425858) to minimize protein A/G contamination or you can use anti-Mouse IgG Agarose beads (GTX27018) instead of Protein A/G.
To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Mouse IgG (Optimized for Prot A/G) (GTXGTX225857-01) or EasyBloker (GTX425858) to minimize protein A/G contamination or you can use anti-Mouse IgG Agarose beads (GTX27018) instead of Protein A/G.