Enterovirus 71 VP1 antibody [GT9512]

Sandwich ELISA detection of recombinant E.coli expressed, full-length Enterovirus 71 VP1 protein (GTX138560-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody (GTX132338)
Pair 2:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody (GTX132339)
Pair 3:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody (GTX132600)

Sandwich ELISA detection of recombinant E.coli expressed, full-length Enterovirus 71 VP1 protein (GTX138560-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody [HL4796] (GTX644808)
Pair 2:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody [HL1929] (GTX637688)

Sandwich ELISA detection of recombinant E.coli expressed, full-length Enterovirus 71 VP1 (GTX138560-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Enterovirus 71 VP1 antibody [HL1929] (GTX637688)
Detection: Enterovirus 71 VP1 antibody [HL4796] (GTX644808) conjugated with HRP
Pair 2:
Capture: Enterovirus 71 VP1 antibody [HL1928] (GTX637687)
Detection: Enterovirus 71 VP1 antibody [HL4796] (GTX644808) conjugated with HRP
Pair 3:
Capture: Enterovirus 71 VP1 antibody [GT9512] (GTX633583)
Detection: Enterovirus 71 VP1 antibody [HL4796] (GTX644808) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT9512] (GTX633583) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant Enterovirus 71 VP1 protein, His tag (GTX138560-pro) (4000-62.5 ng/mL). Coated protein was probed with Enterovirus 71 VP1 antibody [GT9512] (GTX633583) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody [GT9512] (GTX633583) diluted at 1:500.