Fatty Acid Synthase antibody [N1N2], N-term

Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Fatty Acid Synthase antibody detects Fatty Acid Synthase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody (GTX110470) diluted by 1:1000.

Immunoprecipitation of Fatty Acid Synthase protein from HeLa whole cell extracts using 5 μg of Fatty Acid Synthase antibody [N1N2], N-term (GTX110470).
Western blot analysis was performed using Fatty Acid Synthase antibody [N1N2], N-term (GTX110470).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:3000.

Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
5% SDS PAGE
GTX110470 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded OECM1 xenograft, using Fatty Acid Synthase(GTX110470) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Fatty Acid Synthase(GTX110470) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.