GeneTex
GPR30 antibody
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with GPR30 antibody (GTX100001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Due to the high tendency to aggregate, GPR30 samples were not boiled in the SDS gel-loading buffer before electrophoresis.
GPR30 antibody detects GPR30 protein by western blot analysis.
A. 50 μg 293T whole cell lysate/extract
B. 50 μg whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling)
C. 50 μg whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling)
5% SDS-PAGE
GPR30 antibody (GTX100001) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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HostRabbit
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ClonalityPolyclonal
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IsotypeIgG
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ApplicationsWB IHC
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ReactivityHuman, Pig