Gli1 antibody [HL247]

Name: Gli1 antibody [HL247]
Brand: GeneTex
SKU: GTX635619
Price: 419.00 USD

Cat. No. GTX635619

GTX635619 WB Image

Non-transfected (–) and transfected (+) RMS13 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX635619 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL27] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX635619 IP Image

Immunoprecipitation of Gli1 protein from RMS 13 whole cell extract using 5 μg of Gli1 antibody [HL247] (GTX635619).
Western blot analysis was performed using Gli1 antibody [HL247] (GTX635619).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

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GTX635619 WB Image

RMS 13 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX635619 ICC/IF Image

Gli1 antibody [HL247] detects Gli1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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GTX635619 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX635619 IHC-P Image

Gli1 antibody [HL247] detects Gli1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX635619 IHC-P Image

Gli1 antibody [HL27] detects Gli1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Gli1 stained by Gli1 antibody [HL27] (GTX635619) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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This Gli1 antibody [HL247] (GTX635619) is a rabbit monoclonal antibody validated for detection of Gli1 by WB, ICC/IF, IHC-P, and IP. Reactivity has been shown in Human, Mouse, and Rat. Knockout/knockdown validation supports antibody specificity.

Host

Rabbit
Clonality

Monoclonal
Clone Name

HL247
Isotype

IgG
Applications

WB ICC/IF IHC-P IP
Reactivity

Human, Mouse, Rat

Summary

Gli1 antibody detects Gli1 protein, a zinc-finger protein with a predicted molecular weight of 119 kDa. Gli1 is a transcription factor that serves as an effector of Sonic hedgehog signaling in mediating cell fate during embryonic development. In addition, Gli1 has been shown to control many basic cell functions including the cell cycle, growth, and cell death. Although Gli1 was originally isolated from a glioma tumor, its upregulation is found in many other tumor types.