HIF1 alpha antibody [HL3011]

HIF1 alpha antibody [HL3011] detects HIF1 alpha protein by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha stained by HIF1 alpha antibody [HL3011] (GTX640424) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

HIF1 alpha antibody [HL3011] detects HIF1 alpha protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
HIF1 alpha stained by HIF1 alpha antibody [HL3011] (GTX640424) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [HL3011] (GTX640424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [HL3011] (GTX640424) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

HIF1 alpha antibody [HL3011] detects HIF1 alpha protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha stained by HIF1 alpha antibody [HL3011] (GTX640424) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [HL3011] (GTX640424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.