NF-L antibody [DA2]

Sandwich ELISA detection of recombinant full-length NF-L protein using GTX134085 as capture antibody at concentration of 5 μg/mL and GTX24572 as detection antibody was diluted at 1:1000. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of recombinant full-length NF-L protein using GTX101142 as capture antibody at concentration of 5 μg/mL and GTX24572 as detection antibody was diluted at 1:1000. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

IHC-P analysis of human cerebellum tissue using GTX24572 NF-L antibody [DA2]. It labels neuronal cells and their processes.
Antigen retrieval : Heat mediated Citrate buffer (pH6.0).
Dilurion : 1:2000

IHC-P analysis of human cerebellum tissue using GTX24572 NF-L antibody [DA2]. It strongly labels the perikarya and processes of Purkinje cells.
Antigen retrieval : Heat mediated Buffered Tris solution (pH9.5).
Dilurion : 1:2000

IHC (Free Floating) analysis of rat frontal cortex tissue using GTX24572 NF-L antibody [DA2]. It labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the GFAP antibody stains the network of glial cells.
Red : NF-L
Green : GFAP
Dilurion : 1:500

Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including MCA-DA2 (lane 4).

Cells grown from adult rat brain. Large cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin RPCa-a-Int (red). These cells were mitotic but had several characteristics of neurons.