NRF2 antibody [HL1021]

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Immunoprecipitation of NRF2 protein from MG132 treated HepG2 whole cell extract using 5 μg of NRF2 antibody [HL1021] (GTX635826).
Western blot analysis was performed using NRF2 antibody [HL1021] (GTX635826).
EasyBlot HRP-conjugated anti Rabbit IgG (GTX221666-01), and the signal was developed with Trident femto Western HRP Substrate.

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1020] (GTX635826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

NRF2 antibody [HL1021] detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse salivary gland.
NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NRF2 antibody [HL1021] detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

NRF2 antibody [HL1021] detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NRF2 antibody [HL1021] detects NRF2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
NRF2 stained by NRF2 antibody [HL1021] (GTX635826) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (GTX635826) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.