PHGDH antibody [HL6028]

Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHGDH antibody [HL6028] (GTX648102) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PHGDH antibody [HL6028] detects PHGDH protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PHGDH stained by PHGDH antibody [HL6028] (GTX648102) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield™ with DAPI (GTX30920).

PHGDH antibody [HL6028] detects PHGDH protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
PHGDH stained by PHGDH antibody [HL6028] (GTX648102) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PHGDH antibody [HL6028] (GTX648102) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHGDH antibody [HL6028] (GTX648102) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data are based on Human Protein Atlas program.