Procalcitonin antibody [HL3769]

Specificity test was performed by coating the plate with recombinant procalcitonin, calcitonin, and katacalcin proteins (2000 ng/mL). Coated protein was probed with Procalcitonin antibody [HL3769] (GTX641968) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with Procalcitonin antibody [HL3769] (GTX641968) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant Human Procalcitonin, His tag (GTX138116-pro) (0.67-43.1 nM). Coated protein was probed with Procalcitonin antibody [HL3769] (GTX641968) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Procalcitonin antibody [HL3769] detects Procalcitonin protein by immunofluorescent analysis.
Sample: TT cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Procalcitonin stained by Procalcitonin antibody [HL3769] (GTX641968) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Procalcitonin antibody [HL3769] (GTX641968) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.