Rad51 antibody [N1C2]

Cat. No. GTX100469
63
Cat. No.  GTX100469
KO/KD Orthogonal
Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
GTX100469 WB Image
KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
GTX100469 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
GTX100469 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
GTX100469 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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Anti-Rad51 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100469
GTX100469 IHC-P Image

Rad51 antibody [N1C2] detects Rad51 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Rad51 stained by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
GTX100469 WB Image
Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
GTX100469 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000.

7 / 12
Anti-Rad51 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100469
GTX100469 IHC-P Image

Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Rad51 stained by Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
GTX100469 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX100469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-Rad51 antibody [N1C2] used in Immunoprecipitation (IP). GTX100469
GTX100469 IP Image

Rad51 antibody [N1C2] immunoprecipitates Rad51 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Rad51 protein by 4 μg Rad51 antibody [N1C2] (GTX100469)
5 % SDS-PAGE
The immunoprecipitated Rad51 protein was detected by Rad51 antibody [N1C2] (GTX100469) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

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Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
GTX100469 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GTX100469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
GTX100469 WB Image
KO/KD

The data was published in the journal PLoS One in 2013. PMID: 23724141

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Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
Anti-Rad51 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100469
Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
Anti-Rad51 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX100469
Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
Anti-Rad51 antibody [N1C2] used in Immunoprecipitation (IP). GTX100469
Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
Anti-Rad51 antibody [N1C2] used in Western Blot (WB). GTX100469
This Rad51 antibody [N1C2] (GTX100469) is a rabbit polyclonal antibody validated for detection of Rad51 by WB, IHC-P, and IP. Reactivity has been shown in Human, Mouse, Rat, and other species. This product is cited in 63 published studies. Knockout/knockdown validation supports antibody specificity.
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB IHC-P IP
  • Reactivity
    Human, Mouse, Rat, Zebrafish
Summary
RAD51 antibody detects RAD51 protein, also known as RAD51 homolog A. RAD51 (37 kDa) is the indispensable strand exchange factor for homologous recombination in DNA repair. It is also essential for replication fork processing and for genome stability. This RAD51 antibody is a well-cited rabbit polyclonal validated for many applications.
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