Rhodopsin antibody [HL2668]

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:5000 and competitor's antibody (Clone 1D4) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Boiled and unboiled mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:1000 and competitor's antibody (Clone 1D4) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Rhodopsin antibody [HL2668] (GTX639332) detects Rhodopsin protein by flow cytometry analysis.
Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:50 or a Rabbit monoclonal IgG isotype control at 4ºC for 1 hour.

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Rhodopsin stained by Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rat tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Rhodopsin stained by Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Boiled and unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (GTX639332) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.