Synaptotagmin 1 antibody [HL1654]

Cat. No. GTX637252
1 1
Cat. No.  GTX637252
Recombinant antibody KO/KD Orthogonal Overexpression Carrier-Free
Anti-Synaptotagmin 1 antibody [HL1654] used in IHC (Paraffin sections) (IHC-P). GTX637252
GTX637252 IHC-P Image

Synaptotagmin 1 antibody [HL1654] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
GTX637252 WB Image
Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
GTX637252 WB Image

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
GTX637252 WB Image
Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

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Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
GTX637252 WB Image

Human tissue extract (5 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
GTX637252 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (GTX637252) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-Synaptotagmin 1 antibody [HL1654] used in IHC (Paraffin sections) (IHC-P). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
Anti-Synaptotagmin 1 antibody [HL1654] used in Western Blot (WB). GTX637252
This Synaptotagmin 1 antibody [HL1654] (GTX637252) is a rabbit monoclonal antibody validated for detection of Synaptotagmin 1 by WB and IHC-P. Reactivity has been shown in Human, Mouse, Rat, and other species. Knockout/knockdown validation supports antibody specificity.
  • Host
    Rabbit
  • Clonality
    Monoclonal
  • Clone Name
    HL1654
  • Isotype
    IgG
  • Applications
    WB IHC-P
  • Reactivity
    Human, Mouse, Rat, Drosophila
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