alpha Smooth Muscle Actin antibody [HL3088]

alpha Smooth Muscle Actin antibody [HL3088] detects alpha Smooth Muscle Actin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [HL3088] (GTX640534) diluted at 1:5000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Smooth Muscle Actin antibody [HL3088] detects alpha Smooth Muscle Actin protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse duodenum.
Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [HL3088] (GTX640534) diluted at 1:250.
Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:1000.
Blue: Hoechst 33342 staining.

alpha Smooth Muscle Actin antibody [HL3088] detects alpha Smooth Muscle Actin protein by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min.
Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [HL3088] (GTX640534) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [HL3088] (GTX640534) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

alpha Smooth Muscle Actin antibody [HL3088] detects alpha Smooth Muscle Actin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [HL3088] (GTX640534) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Smooth Muscle Actin antibody [HL3088] detects alpha Smooth Muscle Actin protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [HL3088] (GTX640534) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [HL3088] (GTX640534) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.