proBDNF antibody

IHC-Fr analysis of rat hippocampus dentate gyrus tissue using GTX04075 proBDNF antibody.
Panel A : BDNF staining (red) appears in interneuron outlines in the hilus (H) region and in the granule layer (G).
Panel B : proBDNF staining (GTX04075, green) in the same section appears in interneuron outlines in the hilus (H) region and in the granule layer (G).
Dilution : 1:200

WB analysis of various cell lysates using GTX04075 proBDNF antibody.
Lane 1 : C6 cell lysates
Lane 2 : SH-SY5Y cell lysates
Lane 3 : C6 cell lysates treated with blocking peptide
Lane 4 : SH-SY5Y cell lyates treated with blocking peptide
Dilution : 1:200

WB analysis of recombinant proteins using GTX04075 proBDNF antibody.
Lane 1 : 20 ng Human proBDNF
Lane 2 : 200 ng Human proNGF
Lane 3 : 200 ng proNT-3
Lane 4 : 200 ng Human BDNF
Lane 5 : 200 ng Native mouse NGF 2.5S protein
Lane 6 : 200 ng Human Neurotrophin-3 (NT-3)
Dilution : 1:400

IHC-Fr analysis of rat cerebellum tissue using GTX04075 proBDNF antibody. proBDNF (Red) appears in Purkinje cell bodies (vertical arrows) and in astrocytic processes (horizontal arrows in panel A) but not in Purkinje neuronal dendrites stained for calbindin D28k (Green, in panel B) in the same brain section.