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Summary This antibody is expected to recognise all reported isoforms (NP_065151.1, NP_127501.1, NP_127502.1 and NP_127503.1).

Summary Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein.