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Summary The monoclonal antibody JC4 is specific for Cytotoxic necrotizing factor type 1 and the highly related Cytotoxic necrotizing factor type 2 (CNF1 and CNF2) of uropathogenic Escherichia coli. Monoclonal antibody JC4 recognizes an epitope between amino acids 169 to 191 of the N-terminal binding domain. JC4 neutralizes only CNF1.

Summary This antibody is reactive with isolates fixed by phenol, formalin and boiling.

Summary Reacts with the “O” antigen of E. coli serotype O157. Crossreacts with Salmonella O30 (Hilversum) andSalmonella O30 (Urbana). Non-reactive with E. coli serotypes O111, O125, O20 and O55.

Summary Reacts with the “O” antigen of E. coli serotype O157. Crossreacts with Salmonella O30 (hilversum) andSalmonella O30 (Urbana). Non-reactive with E. coli serotypes O111, O125, O20 and O55.

Summary Reacts with the “O” antigen of E. coli serotype O157. Crossreacts with Salmonella O30 (hilversum) andSalmonella O30 (Urbana). Non-reactive with E. coli serotypes O111, O125, O20 and O55.

Summary Reacts with the “O” antigen of E. coli serotype O157. Crossreacts with Salmonella O30 (hilversum) andSalmonella O30 (urbana). Non-reactive with E. coli serotypes O111, O125, O20 and O55.

Summary The monoclonal antibody NG8 is specific for Cytotoxic necrotizing factor type 1 (CNF1) of uropathogenic Escherichia coli. Monoclonal antibody NG8 recognizes an epitope between amino acids 704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.