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Summary Clone CM1 , M9E7, M8C9 bind with high efficiency with different regions of GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule. These antibodies react with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids (T47D cells) or as a result of chemical treatment (NaJO4) of human milk MUC1. They do not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific antibodies and belong to monoclonal antibody cluster 1-4 according to ISOMB TD-4 classification. No cross-reactivity with egg white avidin.

Summary Multiple isoforms of MUC1 are known to exist. MUC1 often migrates at a higher molecular weight in SDS-PAGE due to high levels of post-translational modification.

Summary In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats.

Summary Smad2/3